Raw Message Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin.

Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles.

Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited.

If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. Raw Message Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it.

CBG - 2.5 Gallon (10 Liter) - Benchtop Supreme Solvent Recycler by CBG Biotech/CBG Technologies. Recycles xylene, xylene substitutes, alcohol and acetone with 90-95%. CBG operates eight offices. Cbg Biotech Slv-99ul Solvent Recycler Lab Xylene Alcohol Distillation Purifier. Download Cbg Biotech Solvent Recycler Manual free.

If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full.

We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of ***@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: ***@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin.

Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated.

Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. Raw Message Liz, I am curious, why do you not use the recycled xylene prior to the paraffin step in the processor?

And why not for cleaning? I understand not recycling the cleaning alcohol or xylene, or even the first xylene on the stainer as they have too many contaminants. Nirmala, With regards to getting a lower quality of your alcohol, do you check the water content prior to recycling? We have found that it's not worth attempting to recycle any graded alcohols below 95% (that is to say, 70% or 80%). There is a 'dilute' alcohol setting that can be used, but you will need the CBG folks to tell you how to access that one. It's there though! Michelle -----Original Message----- From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, May 04, 2010 11:44 AM To: ***@mail.holyname.org; ***@lists.utsouthwestern.edu Subject: RE: [Histonet] CBG BIOTECH RECYCLER Nirmala We have the CBG recycler also.

We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning.

It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it.

We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of ***@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: ***@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol.

We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in.

In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles.

Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated.

Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list ***@lists.utsouthwestern.edu _______________________________________________ Histonet mailing list ***@lists.utsouthwestern.edu No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2852 - Release Date: 05/03/10 18:27:00.

Raw Message We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together. Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products.

Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors.

The waste we end up with that cannot be recycled goes in the 55 gal. Barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP). Raw Message Susan, A few questions/comments: 1) how do you test for the xylene for purity? 2) we used to make different batches of 95/100 and 70/80%, but we gave up on that!

It was too much effort for us. We do well with the 95/100 though.

We generally will get 98% - 99% on ours. 3) re: alcohol contaminated with xylene, yes, we do the same thing. On our stainer, we will discard the alcohol after deparaffinization, but keep the alcohol prior to the xylene at the end of the staining run.

Pentax Digital Still Camera Installation Cd. 4) are you hauling off both alcohol and xylene waste? I have found I have very little waste xylene.

Michelle -----Original Message----- From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Susan Raibley Sent: Tuesday, May 04, 2010 10:30 PM To: ***@lists.utsouthwestern.edu Subject: [Histonet] Re: CBG BIOTECH RECYCLER We use the CBG Biotech Recycler to recycle formalin, alcohol and xylene and they all turn out very nicely. We always assay/test each batch to make sure it is ok to use. The xylene always comes out at 99.9% and we use it on all stations of our processor and to stain with and have no problems. We collect our waste alcohols as batches of 70% and 80% to be recycled together and then 95% and 100% to be recycled together.

Ususally we get back between 83% and 98% from the reclaimed alcohol and dilute accordingly. We were told by CBG Biotech Tech Support, there is no limit to how many times you can recycle any of the products. Also, you can recycle xylene with paraffin in it, but DO NOT recycle any alcohol contaminated with xylene. We do not recycle any alcohol off the stainer or the cleaning alcohol off the processors. The waste we end up with that cannot be recycled goes in the 55 gal.

Barrels to be hauled off for proper disposal. Hope this helps! Susan Bincsik, HT (ASCP).

Raw Message Michelle, The xylene purity test is a very simple. Using a 100 ml graduated cylinder. You just add 85 ml of recycled xylene making sure the bottom of the meniscus aligns right at the top edge of the 85 ml mark and then 15 ml of distilled water, making sure the bottom of the meniscus aligns with the top edge of the 100 ml mark. Then using your hand as a stopper, invert the graduate and allow mixture to settle and then place back down and record the separation point at the bottom of the meniscus as the separation point.it should be near 15 ml. Then you subtract 15 from the separation point. The remainder plus 0.1 correction factor is the concentration of recoverd xylene. Ex: If your meniscus aligns at 15.5 ml, you take (15.5 - 15) + 0.1 = 0.6 impurities.

Therefore, your concentration of recovered xylene is 99.4%. I can e-mail you this page from the operation manual so you can print it. All of the information on testing/dillution/assaying formalin, xylene and alcohols should be in the operation manual provided with your recycler. There is a very simple to use alcohol dilution chart in there. You can get a free replacement if you need one by contacting CBG Biotech at 1-800-941-9484.

The product # for our manual which has this info is MIS2001. We haul away alcohol and some formalin, but usually not xylene, since we keep recycling it. At least it has been a very long time since we had to.only when we just get too backed up to recycle it. We work in research and sometimes our volumes of formalin are so great, we have to haul some away, but only as a last resort, so we mostly haul our alcohol away. I hope this helps!

Susan Bincsik, HT (ASCP) RE: [Histonet] Re: CBG BIOTECH RECYCLER Wednesday, May 5, 2010 1:12 PM From: ***@imagesbyhopper.com To: ***@lists.utsouthwestern.edu Susan, A few questions/comments: 1) how do you test for the xylene for purity? 2) we used to make different batches of 95/100 and 70/80%, but we gave up on that!

It was too much effort for us. We do well with the 95/100 though. We generally will get 98% - 99% on ours. 3) re: alcohol contaminated with xylene, yes, we do the same thing. On our stainer, we will discard the alcohol after deparaffinization, but keep the alcohol prior to the xylene at the end of the staining run. 4) are you hauling off both alcohol and xylene waste? I have found I have very little waste xylene.

1001 Nights Turkish Serial Movies 2016. Used: An item that has been used previously. The item may have some signs of cosmetic wear, but is fully operational and functions as intended. Brand: CBG Biotech MPN: SLV-99U (L) Model: SLV-99U (L) UPC: Average delivery time is 1 to 2 days delivery may vary depending on product availability.

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